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PRODUCTION OF NEW WAP-8294A CYCLODEPSIPEPTIDES BY THE BIOLOGICAL CONTROL AGENT LYSOBACTER ENZYMOGENES

Frontiers of Agricultural Science and Engineering 2022, Volume 9, Issue 1,   Pages 120-132 doi: 10.15302/J-FASE-2021410

Abstract: This study investigated the production of new analogs of WAP-8294A compounds by the biocontrol agent Lysobacter enzymogenes OH11 through biosynthetic engineering.WAP-8294As are a family of natural cyclic lipodepsipeptides with potent activity against Gram-positiveIn addition, six new analogs of WAP-8294As were identified by UHPLC-HR-MS/MS.This is the first attempt to produce new WAP-8294As in Lysobacter and shows that the

Keywords： biocontrol / biosynthesis / Lysobacter / natural products / WAP-8294A    

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QUANTITATIVE STUDY ON ANTI-PEST ACTIVITY OF NATURAL PRODUCTS BASED ON VISUALIZATION FRAMEWORK OF KNOWLEDGE

Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 2,   Pages 306-332 doi: 10.15302/J-FASE-2023488

Abstract:

● Using visual analysis to predict the trend of natural product pest

Keywords： anti-pest activity     crop protection     insect pest     natural product     visual analysis    

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Compressive behavior and microstructure of concrete mixed with natural seawater and sea sand

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 6,   Pages 1347-1357 doi: 10.1007/s11709-021-0780-2

Abstract: X-ray powder diffraction (XRD) methods were applied to understand the microstructure and hydration products

Keywords： seawater and sea sand concrete     compressive strength     strain field     microstructure     hydration products    

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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 2,

Abstract:

Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene MdDHAR, thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

 

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Development of a dual temperature control system for isoprene biosynthesis in

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 7,   Pages 1079-1089 doi: 10.1007/s11705-021-2088-0

Abstract: Conflict between cell growth and product accumulation is frequently encountered in the biosynthesis ofstrains harboring the isoprene synthetic pathway in the mitochondria, the dynamic control of isoprene biosynthesisprovides a dual temperature control system for yeast engineering that may also be conducive to the biosynthesisof other high-value natural products.

Keywords： transcriptional activator     directed evolution     dynamic control     heat-shock     isoprene    

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THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

Frontiers of Agricultural Science and Engineering   Pages 231-235 doi: 10.15302/J-FASE-2020367

Abstract: Ascorbic acid (AsA, vitamin C) is involved in the regulation of many aspects of plant growth and development. It is an essential micronutrient for humans and can prevent scurvy, maintain the health of gums and blood vessels, reduce the level of plasma cholesterol and enhance the immune systen. Apple cultivars Orin and Guanghui were crossed to obtain a group of hybrid offspring with and without red flesh in the course of assessing apple germplasm resources. Unexpectedly, the red-flesh apples had higher AsA contents than other apples. Further studies showed that the anthocyanin biosynthetic regulator MdMYB1 directly activates the expression of dehydroascorbate reductase gene , thus promoting the activity of the DHAR enzyme and the accumulation of AsA. This finding reveals the mechanism leading to high AsA levels in red-flesh apples and suggests a new idea to cultivate red-flesh apples with high AsA contents and produce AsA efficiently and without pollution.

Keywords： anthocyanin     apples     ascorbic acid     MdMYB1     vitamin C    

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Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 1, doi: 10.1007/s11783-017-0896-8

Abstract: Valerate-hydrolysate enriched culture showed great ability to produce 3HV and 3H2MV. Valerate-hydrolysate enriched culture had more in the community. Mixed iso-/n-valerate was effective at balancing microbial growth and PHAs synthesis. Co-substrates of valerate and propionate raised the fraction of 3HV and 3H2MV. The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HV and 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

Keywords： Polyhydroxyalkanoates (PHAs)     Valerate     Mixed culture     3-hydroxyvalerate (3HV)     Propionate    

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Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 2,   Pages 271-279 doi: 10.15302/J-FASE-2018214

Abstract: This study reports a strain of CCTCC-SBW0162 with potential to enhance biocontrol activity against

Keywords： anthraquinone     Botrytis cinerea     harzianolide     harzianopyridone     molecular docking     Trichoderma harzianum    

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Screening of indicator pharmaceuticals and personal care products in landfill leachates: a case study

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 9, doi: 10.1007/s11783-023-1716-y

Abstract:

● A systematic framework was developed to identify i-PPCPs for landfill leachate.

Keywords： Landfill leachates     PPCPs     Indicator     Screening criteria     Source-specificity    

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Bacteriophages in water pollution control: Advantages and limitations

Frontiers of Environmental Science & Engineering 2021, Volume 15, Issue 5, doi: 10.1007/s11783-020-1378-y

Abstract:

•Phages can be better indicators of enteric viruses than fecal indicator bacteria.

Keywords： Phage     Water pollution monitoring     Harmful bacteria biocontrol     Horizontal gene transfer     Bacterial resistance    

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Characteristics of typical dissolved black carbons and their influence on the formation of disinfection by-products

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 12, doi: 10.1007/s11783-022-1585-9

Abstract:

● The physicochemical and structural properties of DBC were characterized.

Keywords： Dissolved black carbon (DBC)     Chlorine     Chloramine     Disinfection by-products (DBPs)     Disinfection by-products    

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Data quality assessment for studies investigating microplastics and nanoplastics in food products: Are

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 8, doi: 10.1007/s11783-023-1694-0

Abstract:

● Data quality assessment criteria for MP/NPs in food products were

Keywords： Microplastic     Nanoplastic     Food products     Data quality     Human health risk    

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Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 126-132 doi: 10.1007/s11705-016-1597-8

Abstract: A robust and versatile tool for multigene pathway assembly is a key to the biosynthesis of high-valueFunctional isoprenoid biosynthesis pathways containing 4–11 genes with lengths ranging from ~10 to ~22

Keywords： pathway assembly     toolbox     reiterative recombination     S. cerevisiae     biosynthesis    

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Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products

Yong YU,Laosheng WU

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 3,   Pages 475-481 doi: 10.1007/s11783-014-0640-6

Abstract: Endocrine disrupting compounds (EDCs), pharmaceuticals and personal care products (PPCPs) have attracted

Keywords： endocrine disrupting compounds (EDCs)     pharmaceuticals and personal care products (PPCPs)     fish     bisphenol    

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Integrated risk assessment framework for transformation products of emerging contaminants: what we know

Frontiers of Environmental Science & Engineering 2023, Volume 17, Issue 7, doi: 10.1007/s11783-023-1691-3

Abstract:

● A better risk assessment can combine the improved non-target analysis method.

Keywords： Risk assessment frameworks     Transformation products     Emerging contaminants     High-throughput toxicity    

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Title Author Date Type Operation

PRODUCTION OF NEW WAP-8294A CYCLODEPSIPEPTIDES BY THE BIOLOGICAL CONTROL AGENT LYSOBACTER ENZYMOGENES

Journal Article

QUANTITATIVE STUDY ON ANTI-PEST ACTIVITY OF NATURAL PRODUCTS BASED ON VISUALIZATION FRAMEWORK OF KNOWLEDGE

Journal Article

Compressive behavior and microstructure of concrete mixed with natural seawater and sea sand

Journal Article

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Journal Article

Development of a dual temperature control system for isoprene biosynthesis in

Journal Article

THE ANTHOCYANIN BIOSYNTHETIC REGULATOR MDMYB1 POSITIVELY REGULATES ASCORBIC ACID BIOSYNTHESIS IN APPLE

Jianping AN, Xiaofei WANG, Chunxiang YOU, Yujin HAO

Journal Article

Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant

Jiuxiao Hao, Xiujin Wang, Hui Wang

Journal Article

Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

Journal Article

Screening of indicator pharmaceuticals and personal care products in landfill leachates: a case study

Journal Article

Bacteriophages in water pollution control: Advantages and limitations

Journal Article

Characteristics of typical dissolved black carbons and their influence on the formation of disinfection by-products

Journal Article

Data quality assessment for studies investigating microplastics and nanoplastics in food products: Are

Journal Article

Assembly of biosynthetic pathways in

Lidan Ye,Xiaomei Lv,Hongwei Yu

Journal Article

Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products

Yong YU,Laosheng WU

Journal Article

Integrated risk assessment framework for transformation products of emerging contaminants: what we know

Journal Article